Stage 1 Series – Total Marks – 30 / Time Allowed – 1 Hour
Syllabus:
Introduction, Characteristics of enzymes, Mechanism of enzyme action (Catalysis), Inhibitor
I- Multiple Choice Questions.
- Which one is an organic a cofactor?
a. NAD+
b. Mg++
c. heme
d. None - Which one is correct about the enzymes?
a. Increase pH
b. Increase temperature
c. Increase catalysis
d. All are correct - Which one is incorrect?
a. Apoenzyme + Coenzyme = Holoenzyme
b. Fe++ Ion + Polypeptide = Coenzyme
c. Holoenzyme – Apoenyme = Cofactor
d. Apoenzyme + Zn Ion = Holoenzyme - The active site recognizes its substrate due to _?
a. Charge bearing ability
b. Specific shape
c. ATP Utilization
d. All - Which one denature active site of the enzyme?
a. Temperature
b. pH
c. Inhibitors
d. All - The main cause of rejection of lock and key model?
a. Enzyme act as Key
b. Enzymes acts as lock
c. Active site rigidity
d. Speed of enzyme catalyzed reaction - Which one is an example of permanent inhibitor?
a. Succinic acid
b. Malonic acid
c. Cyanides
d. All - Which one is a not related to proenzyme?
a. Pepsinogen
b. Enzyme precursor
c. Inactive enzyme
d. Denatured enzyme - The non-protein part of enzyme?
a. Activator
b. Cofactor
c. Coenzyme
d. All - Which property of enzymes are different from other proteins?
a. Not composed of amino acids
b. Have specific three-dimensional shape
c. Denatured by high temperature
d. Catalytic molecules
II- Short Answer Questions? - Why some enzymes needed a non-protein part for their proper functions?
- Why enzyme require an aqueous media for proper function?
- An enzyme has turnover number 10. How much time is needed to convert 230 substrate into products?
- Write down roles of binding site and catalytic site.
- What is the importance of induce fit model over the lock and key model?
- Why an irreversible enzyme`s effect cannot be neutralized?
- What is the mode of inhibition of malonic acid?
- What causes specificity of an enzymes?
- Give examples of reversible and irreversible inhibitors
- Write down mechanism of inhibition.
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